SubtiBank SubtiBank
hmoA [2019-09-03 11:00:38]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

hmoA [2019-09-03 11:00:38]

heme monooxygenase
locus
BSU_07150
pI
9.13
mw
14.57 kDa
protein length
125 aa Sequence Blast
gene length
378 bp Sequence Blast
function
degradation of heme
product
heme monooxygenase
essential
no
synonyms
yetG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.3|Acquisition of iron] → [category|SW 1.3.3.3|Acquisition of iron / Other]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.5|Iron metabolism] → [category|SW 2.6.5.1|Acquisition of iron / Other]

    • Gene

    Coordinates
    785,113 785,490

    The protein

    Catalyzed reaction/ biological activity

  • binds hemin ''in vitro'' with ~1:1 stoichiometry and degrade hemin in the presence of an electron donor [Pubmed|21873409]
  • heme b + 3 O2 + 3 reduced [NADPH—hemoprotein reductase] --> biliverdin IXα + CO + Fe2+ + H+ + 3 H2O + 3 oxidized [NADPH—hemoprotein reductase] (according to UniProt)

    • Protein family

  • antibiotic biosynthesis monooxygenase family (with [protein|4EE1809AC405170A78F6F8C8C02EEF5CC54DE076|HmoB], according to UniProt)

    • Structure

  • [PDB|1TZ0] (from [gene|search|B. cereus], 57% identity)

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA]
    description
    (based on genomic context) null

    regulatory mechanism

  • [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur]: repression, [Pubmed|21873409], in [regulon|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|Fur regulon]

    • regulation

  • induced by iron limitation ([protein|search|Fur]) [Pubmed|21873409]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B458 (yetG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1457 NBRP B. subtilis, Japan]
  • BKE07150 ([gene|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTCTCACTCCAATCA, downstream forward: _UP4_TAAAAAGCTTGCGCCTTGCT
  • BKK07150 ([gene|A4E1A1FA886980C863919DA011AC1EEDDBD74967|hmoA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07150 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGTCTCACTCCAATCA, downstream forward: _UP4_TAAAAAGCTTGCGCCTTGCT